EzWay Plasmid DNA Kit, Mini

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EzWay Plasmid DNA Kit, Mini

Catalog Number	K11701	K11702
Size	50T	200T
Type	Kit	Kit
Datasheet

Specification

A simple and reliable way to isolate plasmid DNA from E.coli cultures
Spin column format using silica-based membrane
Yielding up to 20ug of high quality plasmid DNA
No phenol/chloroform extraction or ethanol precipitation required
Purification of plasmid DNA in less than 20 minutes
The A260/280 ratio of purified DNA is within the range of 1.8-1.9.

Kit Contents

DNA-prep Columns
1.5 ml Collection Tubes
RNase A1
Suspension Buffer
Lysis Buffer
Neutralization Buffer
Wash Buffer
Elution Buffer

Protocol

Plasmid DNA released from cell lysate treated by bacterial lysis buffer is bound to a silica gel membrane in the presence of high salts after neutralization and centrifugation. Then, the bound plasmid DNA is eluted in elution buffer.

Applications

PCR
Sequencing
Restriction enzyme digestion
In vitro transcription and translation
In vitro transcription and translation
Ligation
Library screening etc.