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EzWay Gel Extraction Kit
Catalog Number K11770 K11770
Size 50T 200T
Type Kit Kit
Datasheet
 
Specification
  • An easy and rapid way to extract DNA fragment up to 8ug from standard and low-melting agarose gel
  • DNA fragments extracted in 15-20 minutes
  • Recovery of fragments in the size range of 100bp-10Kb
  • Recovery: up to 70-80%
  • Spin column format using silica-based membrane
 
Kit Contents
  • DNA-prep Columns
  • 1.5 ml Collection Tubes
  • Extraction Buffer
  • Wash Buffer
  • Elution Buffer
 
Protocol
After addition of extraction buffer to agarose gel slice sample and heating at 50-55şC to melt gel, DNA is bound to a silica gel membrane in the presence of high concentration of chaotropic salt.
The purified DNA product on the membrane is eluted in elution buffer.
 
Applications
  • Fluorescent DNA sequencing
  • Ligation
  • Transformation
  • PCR
  • Micro-injection
  • DNA microarry-based analysis
  • Restriction mapping
  • Labeling etc.