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Tth111 I
Catalog Number K1750143
Size 300U
Type Buffer & Reagent
Datasheet Inquire
 
Recogntion Site
 
Concentration
5-15 units/ul. Assayed on λ DNA (EcoR I digest).
 
Source
Thermus thermophilus 111.
 
Reaction Condition
Buffer B
[6mM Tris-HCl (pH7.5 @ 37°C), 6mM MgCl2, 50mM NaCl, 1mM DTT]
Incubate at 65°C.
 
Storage Condition
  • 10mM Tris-HCl (pH7.4)
  • 300mM KCl
  • 0.1mM EDTA
  • 1mM DTT
  • 0.5mg/ml BSA
  • 50% glycerol
  • Store at -20°C
 
Isoschizomers
Asp I, PflF I.
 
No. of Cleavage Sites
 
Note
Incubation at 37°C results in 10% activity